Changes in Sleep Satisfaction of Korean Adolescents in the Pre- and Post-COVID-19 Eras and Its Effects on Health Behaviors.

Adolescence is a crucial period for cognitive and psychological development and physical maturation. During this period, hormonally influenced circadian rhythms lead to reduced hours of sleep, and it is important to determine whether sleep quality is sufficient for fatigue relief. Non-face-to-face classes during coronavirus disease-2019 (COVID-19) potentially affected adolescents' sleep quality, psychological state, amount of physical activity, smoking, alcohol consumption, and internet (smartphone) use. We investigated the effects of the COVID-19 situation on adolescents' sleep satisfaction and its relation to the aforementioned factors. Data of 109,281 adolescents collected via an online survey, conducted from 3 June 2019 to 12 July 2019 and from 3 August 2020 to 13 November 2020, were analyzed. Health status comparison between the satisfactory and unsatisfactory sleep groups yielded significant results (odds ratio [OR] = 1.10, confidence interval [CI] = 1.04-1.17) for 2020. In both groups, perceived health was worse in 2019 than in 2020 (OR = 2.72, CI = 2.53-2.92). During COVID-19, non-face-to-face classes increased adolescents' sleep satisfaction. Their psychological state improved, while amount of physical activity (muscle-strengthening exercises), average weight, and internet (smartphone) use increased. Smoking and alcohol consumption decreased.

A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings.

Infectious respiratory diseases such as COVID-19 are serious and global concerns from the past to the present. To isolate the spread of infectious diseases even in the absence of a health system, a simple, inexpensive, reliable, sensitive, and selective molecular diagnosis platform for Point of Care Test (POCT) is required. Especially, the nucleic acid extraction step is difficult to perform out of laboratory. Here, we propose a paper-based lysis (PBL) strip for nucleic acid extraction, especially in low-resource settings (LRS). PBL strips are suitable for isolating RNA from viruses with biological interference and inhibitors. We optimized the buffer compositions and membranes of the strip. A simple preparation method using a PBL strip could obtain an eluent for downstream inspection within 20 min. Overall, 104 copies/swaps were detected for 20 min for amplification in combination with Reverse Transcription Loop-Mediated Amplification (RT-LAMP).

Rapid and simple detection of influenza virus via isothermal amplification lateral flow assay.

Respiratory illness caused by influenza virus is a serious public health problem worldwide. As the symptoms of influenza virus infection are similar to those of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, it is essential to distinguish these two viruses. Therefore, to properly respond to a pathogen, a detection method that is capable of rapid and accurate diagnosis in a hospital or at home is required. To satisfy this need, we applied loop-mediated isothermal amplification (LAMP) and an isothermal nucleic acid amplification technique, along with a system to analyze the results without specialized equipment, a lateral flow assay (LFA). Using the platform developed in this study, all processes, from sample preparation to detection, can be performed without special equipment. Unlike existing PCR methods, the nucleic acid amplification can be performed in the field because hot packs do not require electricity. Thus, the designed platform can provide rapid results without the need to transport the samples to a laboratory or hospital. These advantages are not limited to operations in developing countries with poor access to medical systems. In conclusion, the developed technology is a promising tool for infectious disease management that allows for rapid identification of infectious diseases and appropriate treatment of patients.